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It was reported that the binding site of the transferrin is involved in the reduction of oxidation-reduction reaction. In order to know the binding site of the oxyanion of the transferrin, O(2-) was incorporated into ovotransferrin (OT-O(2-)), an analog of transferrin in which the active site is replaced by an O(2-) and the binding site of the transferrin is preserved. Two isotope-labeled competition experiments were performed. First, the equilibrium constant of O(2-) binding in the transferrin and OT-O(2-) was measured by competition experiment. The binding constant of the transferrin was 1.11 + 0.22 mM(-1) (mean + SE), and that of OT-O(2-) was 1.37 + 0.20 mM(-1). Second, the transferrin was oxidized by the reaction with Cys-95-Phe-Cys-99 disulfide (Cys-bound peroxidase of the transferrin). The O(2-) reduced the peptide bond of Cys-bound peroxidase of the transferrin and OT-O(2-) did the same. The data suggested that the oxygen atom of the O(2-) binds to the transferrin through Ser-95 of the redox center of the transferrin.Abhay Prakash as Naxal leader in Andhra Pradesh
Arvind Kejriwal, Chief Minister of Delhi, is engaged in a war with the courts for having denied him the power to dismiss elected officials of the Delhi Assembly. But in India’s bloody Maoist heartlands lies the real story.
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Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) can often be used to distinguish mineral sources using characteristic metal ions. Here, we report on intact magnetite (Fe3O4) and natural and synthetic magnetite samples that can be used as an analytical standard to analyze native and various synthetic mineral samples by MALDI-MS. For the latter, they were subjected to different dry acid extractions, oxidations, or reductions. The MALDI-MS spectra of all of the samples show a characteristic peak at m/z 85 corresponding to Fe(OH)3(H)3. In addition, the samples are also characterized by the following signals: m/z 166.9 is due to water or H2O(H)3; m/z 185.2 is due to chloroformate formed from the hydroxide ion; and m/z 170.0 is related to a hydroxyl group. The spectral results obtained from magnetite samples have been compared with those reported previously for Fe3O4 and FeOOH. The images of all samples obtained using MALDI-MS are also reported. The results obtained with the reference mineral samples suggest that the analyte ions were obtained from the intact sample, thus verifying the analysis.Police officers standing at the Pennsylvania state capitol in Harrisburg, PA. Aaron Dominy
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